Parameter
|
Assays
|
pKa
|
- Potentiometric titration (Sirius GLpKA)
- Diode array detection D-PAS
- Cosolvent system for hydrophobic substances
|
LogP (water / octanole)
|
- Potentiometric titration (Sirius GLpKA)
- Diode array detection D-PAS
- Shake flask
- Shake flask (miniaturized)
|
LogD (buffer / octanole)
|
- Potentiometric titration (Sirius GLpKA)
- Diode array detection D-PAS
- Shake flask
- Shake flask (miniaturized)
|
KIAM
|
- Distribution coefficient between
immobilized artificial membrane and a solution
|
Solubility in water
|
- Nephelometric pH 7.4
- Saturation solubility (shake flask)
- Saturation solubility (shake flask miniaturized)
- BCS solubility
|
Stability in solution
|
- At pH of solution
- In a buffer system
|
Stability in physiological media
|
- Gastrointestinal fluid (USP)
- Chyle (USP)
|
Metabolic stability
|
- Human liver microsomes
- Microsomes of different animals (beagle, rat, mouse)
|
Protein binding
|
- HSA binding (chromatographic)
- Serum protein binding (ultrafiltration, human)
- Serum protein binding (ultrafiltration, other species)
- Protein binding (ultrafiltration, single proteins)
|
Moisture
|
- Loss on drying
- Karl-Fischer titration
|
Characterisation
|
|