Blood-Brain Barrier Model

BildThe blood-brain barrier (BBB) is an active interface between the circulation and the central nervous system (CNS) which restricts the free movement of different substances between the two compartments and plays a crucial role in the maintenance of the homeostasis of the CNS.

The control over drug passage across the complex barriers between the blood and the brain is a challenging goal in drug discovery.

For this need Across Barriers has established and standardized an in vitro model of the blood-brain-barrier which is based on cerebral capillary endothelial cells.


Following questions could be answered with Across Barriers’ Blood-Brain-Barrier models:

  • Before conducting full toxicological studies, how can I investigate the permeation behavior of drugs and their metabolites?
  • Which specific transport mechanisms are involved in the permeation of my new API
  • Does the compound show toxic effects?
  • How can I obtain predictive data for my computational theoretical model?



Primary cell culture of porcine brain endothelial cells

  • Permeability kinetics
  • Efflux investigations (ab, ba)
  • P-glycoprotein inhibition
  • Active transport (4 °C)
  • Influence of excipient / enhancer

Factsheet "Blood-Brain Barrier"