Analytical Systems

HPLC analysis has become a routine technique in the analytical separation of active ingredients and for assays. HPLC methodology now covers a broad range of techniques including the detection of substances at trace levels. The full power of an HPLC system is best tapped by combining it with an advanced detection system such as a mass spectrometer.
Across Barriers offers a full set of advanced analytical tools ranging from classical pharmacopoeia procedures using HPLC-UV to high-throughput methods for routine assays using LC-MS and LC-MS/MS techniques. The coupling of different chromatographic techniques is a particularly effective means of improving the significance and reliability of chromatographic data.

Analytical systems
  • UV/VIS analysis
  • Fluorescence measurements
  • Scientillation measurements
  • Thin-layer chromatography
  • High-pressure liquid chromatography (HPLC)
Coupled analytical
  • HPLC-PDA (photodiode array)
  • HPLC-PDA-FD (fluorescene detection)
Separation techniques
  • Isocratic methods
  • Gradient methods
  • Size exclusion
Analytical services
  • Chromatographic method development and validation
  • Methods indicating stability, complying with the ICH principles (force degradation)
  • Optimization and adaptation of analytical methods and analytical method transfer
  • Bioanalytics
Method development
  • Literature review
  • Define analytical parameters for method development and/or optimization
  • Stability indicating assay method development
  • Chromatographic separation of drug substance and minor components

Analytical Equipment – Chromatography

Total number 11
Software: Empower 2, ChemStation, MassLynx
Central Server with laces

Waters alliance 2690 (2X)
Waters alliance 2695 (2 x)
Waters alliance 2795 (2 x)

Series 1100 (2X)
Series 1200


Detection systems
Photodiode Array Detector: 966, 996, 2996 from Waters, SPD-M10A from Shimadzu
Diode Array Detector: 1200 from Agilent
Fluorescence Detector: 474 from Waters
Dual Detectors: 2487 from Waters (3 x)
Micromass ZQ 2000
Micromass Quattro Micro
Agilent 1200

Factsheet "Analytics"