Parameter | Assays |
pKa
| - Potentiometric titration (Sirius GLpKA)
- Diode array detection D-PAS
- Cosolvent system for hydrophobic substances
|
LogP (water / octanole) | - Potentiometric titration (Sirius GLpKA)
- Diode array detection D-PAS
- Shake flask
- Shake flask (miniaturized)
|
LogD (buffer / octanole)
| - Potentiometric titration (Sirius GLpKA)
- Diode array detection D-PAS
- Shake flask
- Shake flask (miniaturized)
|
KIAM
| - Distribution coefficient between
immobilized artificial membrane and a solution
|
Solubility in water | - Nephelometric pH 7.4
- Saturation solubility (shake flask)
- Saturation solubility (shake flask miniaturized)
- BCS solubility
|
Stability in solution
| - At pH of solution
- In a buffer system
|
Stability in physiological media
| - Gastrointestinal fluid (USP)
- Chyle (USP)
|
Metabolic stability | - Human Liver Microsomes
- Microsomes of different animals (beagle, rat, mouse)
|
Protein binding
| - HSA binding (chromatographic)
- Serum protein binding (ultrafiltration, human)
- Serum protein binding (ultrafiltration, other species)
- Protein binding (ultrafiltration, single proteins)
|
Moisture
| - Loss on drying
- Karl-Fischer titration
|
Characterisation
| |
